Inoculation of seed ,vith bacterial culture

Guar plant develops, nodules on its roots, in which a special kind of bacteria live and convert
the free nitrogen of the atmosphere into a form of fertilizer which is absorbed by the roots of the
plant. This symbiotic relation of the bacteria and root nodules is useful in saving cost of nitrogenous
fertilizers. Therefore, before sowing the seeds are inoculated with these bacteria so that their
population increases in the soil, with the growth of the plant. This is done by preparing a 10% sugar or gur solution in boiling water. This sugar solution is allowed to cool. On cooling 3 -4 packets of guar bacterial culture are mixed with solution to make a thin paste. This paste is coated over to the seed. Seed is dried under shade for 30 -40 minutes before sowing.

Properties of Guar Gum

The most important property of guar gum is its ability to hydrate rapidly in cold water to attain uniform and very high viscosity at relatively low concentrations. Apart from being the most cost-effective stabilizer and emulsifier it provides texture improvement and water binding, enhances mouth feel and controls crystal formation. The main properties of guar gum are:

.It is soluble in hot & cold water but insoluble in most organic solvents. 
.It has strong hydrogen bonding properties. 
.It has excellent thickening, emulsion, stabilizing and film forming properties. 
.It has excellent ability to control rheology by water phase management. 
.The viscosity of guar gum is influenced by temperature, pH, presence of salts and 
other solids. 


Chemically, guar gum is a straight chain galactomannan, which is 75-85% of the endosperm, has a chain of (1→4)-linked-β-D-mannopyranosyl units with single α-D- galactopyranosyl units connected by (1→6) linkages to, on the average, every second main chain unit (Fig. 4). The ratio of D-mannopyranosyl to D-galactopyranosyl units is about 1.8:1. The average molecular weight of guaran is in the range of 1-2 ×106 dalton. The cis-position is important since adjacent hydroxyl groups reinforce each other in hydrogen bonding reactions.